Abnormalities in reparative function of lung-derived mesenchymal stromal cells in emphysema

نویسندگان

چکیده

Mesenchymal stromal cells (MSCs) may provide crucial support in the regeneration of destructed alveolar tissue (emphysema) chronic obstructive pulmonary disease (COPD). We hypothesized that lung-derived MSCs (LMSCs) from patients with emphysema are hampered their repair capacity, either intrinsically or due to interaction damaged microenvironment. LMSCs were isolated lung controls and severe characterized at baseline. In addition, seeded onto control emphysematous decellularized scaffolds assessed for deposition extracellular matrix (ECM). observed no differences surface markers, differentiation/proliferation potential, expression ECM genes between control- COPD-derived LMSCs. Notably, displayed lower FGF10 HGF messenger RNA (mRNA) hepatocyte growth factor (HGF) decorin protein. When on scaffolds, showed engraftment, proliferation, survival within 2 wk, similar ability deposit new scaffolds. Moreover, LMSC numbers not compromised Collectively, our data show COPD compared less mRNA, mRNA protein, whereas other features including various molecules unaffected. Furthermore, capable functioning native The damaged, microenvironment as such does hamper potential Thus, specific intrinsic deficiencies production by diseased contribute impaired emphysema.

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ژورنال

عنوان ژورنال: American Journal of Physiology-lung Cellular and Molecular Physiology

سال: 2021

ISSN: ['1522-1504', '1040-0605']

DOI: https://doi.org/10.1152/ajplung.00147.2020